Figure A1.

Induction of MDSCs is mediated by soluble factor(s) secreted from HSCs in vitro. BM cells and HSCs were cultured in transwell (T) or conventional plates (C). Expression of CD11b and Gr-1 was analyzed through flow cytometry. Double positivity for CD11b and Gr-1 stands for MDSCs (upper panel). The bar graph shows the absolute numbers of CD11b⁺GR-1⁺ cells/well that were calculated and are expressed as the mean ± 1 SD (* p < 0.05; lower panel). Data representative of three separate experiments.