Figure 2.

Cleavage analysis of a panel of 69 recombinant human cytokines and chemokines. The various recombinant cytokines and chemokines were divided into two separate tubes. One was kept as negative control (C) where PBS (no enzyme) was added and one was cleaved with the human tryptase. The cleavage was performed at 37 °C in PBS buffered solution (pH 7.3) for 2.5 h. The samples were separated on 4–12% SDS-PAGE gels under reducing conditions. Size markers are found at the left side of each gel. The gels were stained in colloidal Coomassie blue solution. (a) Cleavage with 13 ng of tryptase. (b) Cleavage using three times higher enzyme concentration. The cytokines and chemokines that were efficiently cleaved or trimmed are marked by red rectangles.