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. 2019 Oct 11;20(20):5041. doi: 10.3390/ijms20205041

Figure 2.

Figure 2

Chromatograms of (a) the saccharides components in maple sap after ultrafiltration, (b) the purified oligosaccharide, and (c) the saccharide components after the hydrolysis of the purified oligosaccharide. The peak (*) was obtained by fractionating and purifying an unidentified oligosaccharide from maple sap. Analytical conditions: apparatus, LC-10AD pump, degasser model DGU-12A and a Corona Veo detector; column, Asahipak NH2P-50 4E (5 µm, 4.6 mm internal diameter × 250 mm); mobile phase, acetonitrile/deionized water (3:1; v/v); flow rate, 1 mL/min; injection volume, 20 µL; column temperature, 25 °C.