Figure A2.

Molecular weight distribution curves of megamers G3^G0 and G3^G02N estimated using GPC experiments using the references obtained by reaction of PAMAM dendrimers G2, G3, and G4 with gl according to the protocol described for G3^gl. Obtained derivatives bear 46, 78, and 140 2,3-dihydroxyproyl substituents and averaged molecular weight 6600, 12,681, and 24,575 Da, respectively for G2^46gl, G3^78gl, and G4^140gl. The stoichiometry of these G2^gl, G3^gl, and G4^gl derivatives was determined using ¹H NMR spectroscopy. The G2^gl, G3^gl, and G4^gl were used as molecular weight standards to determine the molecular weight of megamers. The figure represents normalized weight fraction vs logM (where M—molecular weight). Samples of megamers G3^G0a and G3^G0b were obtained from G3^64gl : 18 NPCF : 20 G0 according to general protocol described in Experimental section. The average molecular weight of the samples G3^G0b and G3^G0a were estimated for conjugate before (b) and after (a) dialysis. Partial loss of G0 from the conjugate upon three days extensive dialysis can be concluded from that experiment (from average 13 to 10).