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. 2019 Oct 22;20(20):5227. doi: 10.3390/ijms20205227

Figure 1.

Figure 1

Characterization of neutrophil-derived microvesicles (NMV) internalization by hCMEC/D3. (A) NMV size distribution were measured using ZetaView nanoparticle tracking analysis. NMV express (B) the neutrophil surface antigen CD66b and (C) annexin V, but not (D) CD14 (monocytes), as detected by flow cytometry. Isotype control was used to gate for CD66b and CD14 positivity, unlabeled NMVs were used to set the gate for Annexin V positivity. Histograms are representative of three individual neutrophil isolations.