Figure 2.

(A) Exemplary retinal flatmounts labeled with Brn3a from control rats and animals, which were immunized with HSP27 after four, five, and six weeks. (B) Mean retinal ganglion cell density of immunized rats after four, five, and six weeks, and control animals were plotted for four different eccentricities from the optic nerve head (1 = closest to optic nerve head). Five and six weeks after immunizations, animals expressed a lower retinal ganglion cell density. (C) At four, five, and six weeks, mean antigen-antibody reactivity of the serum of HSP27 and control animals was plotted against the corresponding molecular weight of the retinal antigen and revealed, e.g., a significant up- and down-regulations of certain antibodies reactivities. Scale bar = 10 µm [46].