Table 1.
List of HCN3 channel-interacting proteins identified by LC/MS/MS during mass spectrometry.
| Protein Name | UniProt Accession | UniProt Entry Name | Gene Name | Molecular Mass (kDa) |
|---|---|---|---|---|
| 40S ribosomal protein S3 * | E9PPU1 | E9PPU1_HUMAN | RPS3 | 17.4 |
| ATP synthase subunit alpha_ mitochondrial * | P25705 | ATPA_HUMAN | ATP5F1A | 59.9 |
| ATP synthase subunit beta_ mitochondrial * | P06576 | ATPB_HUMAN | ATP5F1B | 56.6 |
| ATP-dependent RNA helicase DDX3X * | A0A2R8Y5G6 | A0A2R8Y5G6_HUMAN | DDX3X | 69.4 |
| C-1-tetrahydrofolate synthase_ cytoplasmic * | P11586 | C1TC_HUMAN | MTHFD1 | 102.2 |
| Clathrin heavy chain 2 | A0A087WX41 | A0A087WX41_HUMAN | CLTCL1 | 144.1 |
| Elongation factor Tu_ mitochondrial * | P49411 | EFTU_HUMAN | TUFM | 49.9 |
| Eukaryotic translation initiation factor 4 gamma 1 | Q04637 | IF4G1_HUMAN | EIF4G1 | 176.2 |
| Fatty acid synthase | A0A0U1RQF0 | A0A0U1RQF0_HUMAN | FASN | 275.8 |
| Filamin-A | A0A087WWY3 | A0A087WWY3_HUMAN | FLNA | 248.3 |
| Golgin subfamily B member 1 | Q14789 | GOGB1_HUMAN | GOLGB1 | 377.4 |
| Heat shock 70 kDa protein 1A * | P0DMV8 | HS71A_HUMAN | HSPA1A | 70.3 |
| Heat shock-related 70 kDa protein 2 | P54652 | HSP72_HUMAN | HSPA2 | 70.3 |
| Isoform 2 of Clathrin heavy chain 1 | Q00610-2 | CLH1_HUMAN | CLTC | 189.7 |
| Isoform 2 of Glyceraldehyde-3-phosphate dehydrogenase | P04406-2 | G3P_HUMAN | GAPDH | 31.7 |
| Isoform 2 of Heat shock protein 75 kDa_ mitochondrial * | Q12931-2 | TRAP1_HUMAN | TRAP1 | 74.5 |
| Isoform 3 of Pyruvate kinase PKM | P14618-3 | KPYM_HUMAN | PKM | 56.9 |
| Isoform 3 of Vacuolar protein sorting-associated protein 13C | Q709C8-3 | VP13C_HUMAN | VPS13C | 419.6 |
| Isoform MBP-1 of Alpha-enolase | P06733-2 | ENOA_HUMAN | ENO1 | 37.3 |
| Isoform Short of Delta-1-pyrroline-5-carboxylate synthase * | P54886-2 | P5CS_HUMAN | ALDH18A1 | 87.8 |
| Matrix-remodeling-associated protein 5 | Q9NR99 | MXRA5_HUMAN | MXRA5 | 314.3 |
| Polyadenylate-binding protein 4 | Q13310 | PABP4_HUMAN | PABPC4 | 71.1 |
| Polyubiquitin-B (Fragment) * | J3QS39 | J3QS39_HUMAN | UBB | 10.5 |
| Prohibitin (Fragment) * | C9JZ20 | C9JZ20_HUMAN | PHB | 22.3 |
| Prohibitin-2 * | Q99623 | PHB2_HUMAN | PHB2 | 33.3 |
| Receptor of activated protein C kinase 1 | P63244 | RACK1_HUMAN | RACK1 | 35.5 |
| RuvB-like 2 | Q9Y230 | RUVB2_HUMAN | RUVBL2 | 51.3 |
| Tubulin beta chain | Q5JP53 | Q5JP53_HUMAN | TUBB | 48.2 |
| Tubulin beta-4B chain | P68371 | TBB4B_HUMAN | TUBB4B | 50.3 |
| Vimentin | P08670 | VIME_HUMAN | VIM | 53.7 |
The resulting candidate proteins had a percentage ≥95% of reliability (Protein AutoCurate green), highlighting the robustness of the analytical method. * Proteins identified with at least one Gene Ontology (GO) term related with mitochondria (www.geneontology.org, www.pantherdb.org and www.string-db.org) and these proteins are listed in the actual version of the MitoProteome database (www.mitoproteome.org).