Figure 2.

Specificity of the anti-T<>T Mab, and its optimisation, determined by DDIP-qPCR. DNA immunoprecipitation was performed using the MeDIP kit from Diagenode, with commercially available, extracted, human DNA irradiated with 0, 0.1, 0.2 and 0.5 J/cm² UVB, and a monoclonal antibody against thymine dimers (T<>T). (A) ELISA results demonstrating the specificity of the anti-T<>T Mab for UV-modified DNA. Quantitative PCR was performed using primers specific for (B) the GAPDH gene promoter, an actively expressed gene, and (C) the Myoglobin exon 2, an inactive gene. Recovery was expressed as a percentage of the amount of immunoprecipitated DNA compared to the input DNA after qPCR. The results are presented as the mean ± SEM of three independent experiments. **** represents p < 0.001.