Figure 5.

Carvedilol increased ABCA1 transcription, but not ABCA1 protein degradation, in THP-1 macrophages. (A) THP-1 macrophages were treated with various doses of Cav for 24 h. The mRNA levels of LXRα, SMPDL3A, and GAPDH were analyzed by qRT-PCR. The relative expressions of mRNA level are presented. (B) THP-1 cells were treated with or without Cav (10 μM) in the presence of cycloheximide (CHX, 2 μg/mL). Cell lysates were collected at the indicated time points, and the expressions of ABCA1 and actin were determined. The relative expression to each control group (with/without CHX) is presented. (LXR, liver X receptor; SMPDL3A, sphingomyelin phosphodiesterase acid like 3A).