Fig 2. sHsps differ in ability to form assemblies with substrate.
(A) DLS measurements of sHsps-luciferase assemblies. Average hydrodynamic radius (±SD) of the most occupied peak (min. 80% of total volume) from DLS size distributions of sHsps-luciferase assemblies. sHsps-luciferase assemblies were prepared as follows: luciferase at fixed 1.5 μM concentration in the presence of sHsps (0 to 10 μM as depicted) was denatured at 44°C for 10 min. When IbpAEc and IbpBEc were tested together (IbpABEc), the 1:2 stoichiometry was used (e.g. 10 μM IbpABEc is 3.33 μM IbpAEc and 6.67 μM IbpBEc). (B) Sedimentation profiles of sHsps-luciferase assemblies. 1 μM luciferase was denatured at 44°C for 10 min in the presence of 5 μM sHsps and subjected to sedimentation in glycerol gradient. Fractions were collected from the top and analysed by SDS-PAGE followed by Oriole staining.