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Luciferase (1.5 μM) was denatured in the presence of 10 μM sHsps (IbpABEc stoichiometry as in Fig 2A), diluted to 60 nM and refolded at limiting (DnaK 0.7 μM; DnaJ 0.28 μM; GrpE 0.21 μM) or 5x higher saturating (DnaK 3.5 μM; DnaJ 1.4 μM; GrpE 1.05 μM) Hsp70 machinery concentrations and ClpB at 2 μM concentration. Data are the mean ± SD of three independent experiments.
