Fig 7. Expressing BON1 in guard cells reduces autoimmunity in bon1-1.

(a) Growth of Pst DC3000 at 0 dpi and 3 dpi after dipping or syringe-infiltration. Results are from three biological repeats in one experiment representative of two independent experiments. Error bars indicate SDs (n = 3). Different letters indicate statistically significant differences in pathogen growth between different genotypes (p< 0.05, student’s t test). (b, c) Transcript abundance of PR1 (b) and SNC1 (c) in mesophyll cells and whole leaves assayed by quantitative Real-time PCR (qPCR). Plants were grown for 5 weeks at 22°C and RNA was harvested from mesophyll cells or rosette leaves, respectively. Results represent three biological replicates. The expression of target genes was normalized to reference gene ACTIN, and relative to their expression in whole leaves of Col-0 which was set as 1. Values are arithmetic means ± standard error (S.E.). Different letters indicate statistically significant differences between indicated plant lines (p< 0.05, based on one-way ANOVA followed by Duncan’s new multiple range test). (d) Growth phenotype of 4-week-old plants from the T2 generation of pGC1::BON1/bon1 transgenic line grown at 22°C, 12h/12h L/D. White arrows point to the flat or twisted young leaves of the T2 plants with (left panel) or without (right panel) the pGC1::BON1 transgene respectively (Scale bar = 1 cm).