Table 2. Mutations identified in selected populations.

Strain	Gene	Pop (%)	Clonal	Description
Dap1	rpsJ	100.0	✓	30S ribosomal protein S10
	Intergenic EF_0871/EF_0872	41.4
	comG1	100.0
	EF_3010	100.0
Amp4	pyrR	43.0
	hexA	31.7
	Intergenic EF_3191/EF_3192	100.0	✓
	EF_3290	93.0	✓	Sensor histidine kinase
Ox1	EF_1820	40.0
	EF_3290	40.0		Sensor histidine kinase
Dox4	rpsJ	87.0	✓	30S ribosomal protein S10
	rpsJ	100.0	✓	30S ribosomal protein S10
	prgB	100.0	✓
Spt4	rpsE	100.0	✓	40S ribosomal protein S5
Lzd2	EF_0149	100	✓
	23S ribosomal RNA	66.2	✓	23S ribosomal RNA
Cip1	Intergenic EF_0115/EF_0116	30.5
	Intergenic EF_1077/EF_1078	100.0	✓
	parC	100.0	✓	Topoisomerase IV
	EF_2485	40.9
	nusG	45.6
	EF_2981	44.3
Lvx3	gyrA	100	✓	DNA gyrase
	gyrA	100	✓	DNA gyrase
	parC	100	✓	Topoisomerase IV
	EF_1692	100	✓

Check marks indicate that the same mutation was also identified in clonal isolates from the same population.

Abbreviations: Amp, ampicillin; Cip, ciprofloxacin; Dap, daptomycin; Dox, doxycycline; gyrA, DNA gyrase subunit A; hexA, DNA mismatch repair protein hexA; Lvx, levofloxacin; Lzd, linezolid; nusG, transcription termination/antitermination protein nusG; Ox, oxacillin; parC, DNA topoisomerase 4 subunit A; Pop, population; pyrR, bifunctional protein PyrR (pyrimidine operon regulatory protein); rpsE, 30S ribosomal protein S5; rpsJ, 30S ribosomal protein S10; Spt, spectinomycin