Table 2.
Anatomical and echocardiographic parameters 8 weeks after induction of pressure overload in Sirt1f/f and Sirt1ciKO mice. BW, Body weight. TL, Tibia length. HW, Heart weight. HW/BW, Heart weight-to-body weight ratio. HW/TL, Heart weight-to-tibia length ratio. LW/BW, Lung weight-to-body weight ratio. LW/TL, Lung weight-to-tibia length ratio. KW/BW, Kidney weight-to-body weight ratio. KW/TL, Kidney weight-to-tibia length ratio. HR, Heart rate. IVSd, Interventricular septal thickness at end-diastole. IVSs, Interventricular septal thickness at end-systole. LVIDd, Left ventricular internal dimension at end-diastole. LVIDs, Left ventricular internal dimension at end-systole. LVWPd, Left ventricular posterior wall thickness at end-diastole. LVWPs, Left ventricular posterior wall thickness at end-systole. EDV, Left ventricular telediastolic volume. ESV, Left ventricular telesystolic volume. LVEF, Left ventricular ejection fraction. LVFS, Left ventricular fractional shortening. SV, Stroke volume. LV, left ventricular mass. CO, Cardiac output. ANOVA: ¶ p ≤ 0.05, ¶¶ p ≤ 0.01, ¶¶¶ p ≤ 0.001 for the genotype effect; § p ≤ 0.05, §§ p ≤ 0.01, §§§ p ≤ 0.001 for the TAC effect; i p ≤ 0.05, ii p ≤ 0.01 for the interaction effect. Post hoc Newman–Keuls test: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 Sirt1f/f vs. Sirt1ciKO (same surgery); $ p ≤ 0.05, $$ p ≤ 0.01, $$$ p ≤ 0.001 Sham vs. TAC (same genotype).
| Sirt1f/f | Sirt1ciKO | 2 Way ANOVA | |||
|---|---|---|---|---|---|
| SHAM | TAC | SHAM | TAC | ||
| BW (g) | 28.3 ± 0.4 | 28.5 ± 0.7 | 28.7 ± 0.5 | 28.7 ± 0.8 | / |
| TL (mm) | 17.6 ±0.1 | 17.8 ± 0.1 | 17.6 ± 0.1 | 18.2 ± 0.1 | / |
| HW (mg) | 153.4 ± 4.4 | 217.7 ± 12.8 $ | 158.9 ± 4.0 | 249.6 ± 24.7 $$$ | §§§ |
| HW/BW (mg/g) | 5.4 ± 0.1 | 7.6 ± 0.3 $$$ | 5.5 ± 0.1 | 8.7 ± 0.7 $$$,* | §§§ |
| HW/TL (mg/mm) | 8.7 ± 0.2 | 12.2 ± 0.7 $$ | 9.0 ± 0.3 | 13.7 ± 1.3$$$ | §§§ |
| LW/BW (mg/g) | 5.2 ± 0.1 | 5.4 ± 0.2 | 5.1 ± 0.1 | 6.2 ± 0.9 | / |
| LW/TL (mg/mm) | 8.3 ± 0.2 | 8.7 ± 0.5 | 8.4 ± 0.2 | 9.9 ± 1.6 | / |
| KW/BW (mg/g) | 12..3 ± 0.3 | 11.2 ± 0.3 $ | 12.1 ± 0.2 | 11.5 ± 0.3 | § |
| KW/TL (mg/mm) | 19.8 ± 0.6 | 17.9 ± 0.6 | 19.7 ± 0.6 | 18.2 ± 0.7 | § |
| HR (bpm) | 542 ± 19 | 541 ± 13 | 514 ± 23 | 543 ± 14 | / |
| IVSd (mm) | 0.72 ± 0.05 | 0.93 ± 0.07 | 0.71 ± 0.04 | 0.83 ± 0.04 | §§ |
| IVSs (mm) | 1.36 ± 0.05 | 1.39 ± 0.07 | 1.34 ± 0.03 | 1.21 ± 0.04 * | / |
| LVIDd (mm) | 3.79 ± 0.09 | 4.03 ± 0.17 | 3.88 ± 0.1 | 4.14 ± 0.11 | § |
| LVIDs (mm) | 2.11 ± 0.08 | 2.79 ± 0.16 $$$ | 2.24 ± 0.08 | 3.19 ± 0.11 $$$,* | ¶, §§§ |
| LVPWd (mm) | 0.71 ± 0.03 | 0.81 ± 0.08 | 0.67 ± 0.03 | 0.87 ± 0.04$ | §§ |
| LVPWs (mm) | 1.25 ± 0.04 | 1.23 ± 0.06 | 1.21 ± 0.04 | 1.14 ± 0.02 | / |
| EDV (mL) | 0.140 ± 0.009 | 0.152 ± 0.014 | 0.149 ± 0.011 | 0.179 ± 0.012 | / |
| ESV (mL) | 0.026 ± 0.003 | 0.061 ± 0.011 $$ | 0.031 ± 0.003 | 0.086 ± 0.008 $$$,* | ¶, §§§ |
| LVEF (%) | 81.7 ± 0.9 | 65.0 ± 2.2 $$$ | 79.5 ± 1.1 | 52.5 ± 2.4 $$$,*** | ¶¶¶, §§§, ii |
| LVFS (%) | 44.5 ± 1.0 | 30.8 ± 1.4 $$$ | 42.4 ± 1.0 | 23.0 ± 1.3 $$$,*** | ¶¶¶, §§§, i |
| SV (mL) | 0.11 ± 0.1 | 0.11 ± 0.01 | 0.12 ±0.01 | 0.09 ± 0.01 | / |
| LV (mg) | 92 ± 4 | 136 ± 17 $$ | 91 ± 5 | 135 ± 10 $$$ | §§§ |
| CO (mL/min) | 61.3 ± 3.9 | 54.3 ± 5.3 | 60 ± 4.6 | 50.8 ± 4 | / |