Analysis of key outcome measures in FLS from a set of healthy donors, treated for 6 days with IL-1α (IL-1), TNF-α (TNF), or cartilage wear particles (CWP), with untreated controls (CTL). Similar trends were observed in response to cytokine and particle treatment for monolayer content of DNA (a), and release of hyaluronan (b), inflammatory stress markers (eg. PGE2, c), cytokines (eg. IL-6, d), and MMPs (eg. MMP-1, e). N = 3 donors. *p < 0.05 vs. CTL. **p < 0.05 vs. CTL and CWP.