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. 2019 Nov 6;5(11):eaay1971. doi: 10.1126/sciadv.aay1971

Fig. 2. CBP–α-TNF accumulated in the inflamed paw.

Fig. 2

Arthritis (CAIA) was induced selectively in the right hind paw by passive immunization of anticollagen antibodies, followed by subcutaneous injection of LPS at the right hind footpad and phosphate-buffered saline at the left hind footpad. On the day following LPS injection, Cy7-labeled CBP–α-TNF and Cy7-labeled α-TNF were intravenously injected into naïve and CAIA mice. Representative images of accumulation in arthritic or nonarthritic paws of mice injected with CBP–α-TNF (A) and α-TNF (B) are shown. (C) Changes in radiant efficiency ratio of the arthritic paw (right hind) to the nonarthritic paw (left hind) in naïve and CAIA mice (n = 3 to 4, means ± SD). *P < 0.05 compared with the area under the radiant efficiency ratio-time curve from 0 to 72 hours of each treatment group (Tukey’s multiple comparison test). (D) Representative histology images of joints in CBP–α-TNF–injected CAIA mouse [left, hematoxylin and eosin (H&E) staining; right, immunohistochemistry staining against anti-rat immunoglobulin G (IgG)]. Scale bars, 200 μm.