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. 2019 Oct 20;11(20):8745–8759. doi: 10.18632/aging.102249

Figure 6.

Figure 6

miR-221 inhibited proliferation and fibrosis-related proteins by targeting SIRT1. (A) Directly binding region between miR-221 and SIRT1; (B) Binding relationship between miR-221 and SIRT1 was detected by luciferase reporter assay with wild or with the mutant type of the luciferase reporter plasmids of SIRT1; (C–H) qPCR and western blot assays revealed that mimic-miR-221 inhibited SIRT1 expression, and inhibitor-miR-221 enhanced SIRT1 expression; (I) The efficiency of siRNA-SIRT1 was measured in MCs by qPCR; (J–K) Protein expression level of Col-4 was measured by western blot in the MCs, which were transfected with inhibitor-miR-221 or siRNA-SIRT1; (L–M) protein expression level of p53 was measured by western blot in the MCs, which were transfected with inhibitor-miR-221 or siRNA-SIRT1. *P < 0.05, **P < 0.01.