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. 2019 Oct 22;11(20):8998–9012. doi: 10.18632/aging.102366

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Copyright © 2019 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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TEPs promote CRC cell proliferation and inhibit cancer cell apoptosis. (A–B) The protein level of TIMP1 in platelets from HV and CRC by ELISA (A) and western blotting (B). (C–D) The mRNA (C) and protein (D) level in HT29 cells incubated with platelets from CRC patients and HVs. (E) The proliferation of HT29 cells exposed to platelets from HVs and colorectal cancer patients were determined by CCK-8 assays. (F) Apoptosis of HT29 cells exposed to platelets from healthy volunteers and colon cancer patients for 72 h were determined by flow cytometry. (G–H) The mRNA (G) and protein (H) level in Caco-2 cells incubated with platelets from CRC patients and HVs. (I) The proliferation of Caco-2 cells exposed to platelets from HVs and colon cancer patients were determined by CCK-8 assays. (G) Apoptosis of Caco-2 cells exposed to platelets from HVs and colon cancer patients for 72 h were determined by flow cytometry. **P < 0.01, ***P < 0.001.