Figure 1.

Monocytes/macrophages secrete a higher amount of 27-HC. A. Immunohistochemical staining of CD11b was determined in 4T1-derived breast tumor tissues. B. A meta analyses of macrophage infiltration were performed by chi-square test with 652 breast tumor samples and 114 normal breast tissue samples from TCGA database. C. Secretion of 27-HC was measured by 27-HyCL ELISA kit in breast cancer cells such as MCF-7 and MDA-MB-231 (231), THP-1 cells, and THP-1-derived macrophages (TDM, THP-1 stimulated with 100 nM PMA). *P < 0.05, **P < 0.01 vs MCF-7 or 231, n = 3, the present data are mean ± SEM. D. Expressions of CYP27A1 in different type macrophages in enriched and non-enriched group breast cancer samples in TCGA database.