Figure 3.

THP-1 cells were polarized to M2 type macrophages by breast cancer media. A. Morphological changes of THP-1 cells were examined when the cells were co-cultured with MCF-7 or MDA-MB-231 for 48 hours. B. HE staining of THP-1 cells was performed under the condition of co-culture, supernatant stimulation, or transwell culture for 48 hours. C. The expressions of CD11b and MMP-9 were detected by western blot analysis in MCF-7 (M), MDA-MB-231 (231) and THP-1 (T) cells differentiated by PMA and conditional media or not. D. The mRNA expressions of CD14, CD25, CD163, IL-10 were detected by q-PCR in THP-1 cells differentiated by conditioned or control media. Human GAPDH was used as housekeeping control gene. E. CD163 expression in THP-1 cells was analyzed using Flow cytometry with or without stimulation of the conditioned media.