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. 2019 Oct 1;9(10):2194–2208.

Figure 4.

Figure 4

M2 type macrophages are the major sources of 27-HC production. A. Schematic drawing for differentiation of mouse primary bone marrow cells by breast cancer media. B. The expression of CD206 was examined by western blot in bone marrow and differentiated cells. C. The mRNA expression levels of M1 type and M2 type marker genes were detected by q-PCR in bone marrow cells differentiated by M-CSF and 4T1CM. D. The expression of CD206 was detected by western blot in peritoneal macrophages stimulated with LPS or IL-4 for 48 hours. E. Comparison of CYP27A1 expression was performed by western blot in 4T1 cells and different subtypes of macrophages. F. Secretion of 27-HC was measured in mouse peritoneal macrophages stimulated with LPS or IL-4 for 48 hours. The values represent the mean ± SEM, each experiment was repeated at least three times. G. Expression of CYP27A1 was determined by western blot in THP-1 cells stimulated with PMA or conditional media from MCF-7 and MDA-MB-231.