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. 2019 Oct 1;9(10):2140–2155.

Figure 4.

Figure 4

25-HC enhances the activation of multiple signaling pathways. A. HepG2 cells were treated with the indicated concentrations of 25-HC for 24 hours or treated with 25-HC at 10 μM for the indicated times before protein was collected to detect the p-Erk1/2, Erk1/2, p-p38, p38, p-SAPK/JNK, SAPK/JNK, p-Akt, Akt, p-PI3K p85/p55, PI3K p85, p-Stat3, Stat3, p-NF-κB p65, NF-κB p65 expressions by Western blotting. B. HepG2 cells were transfected with pNF-κB-luc, pAP-1-luc and pSTAT3-TA-luc reporter plasmids for 24 hours before cells were treated with the indicated concentrations of 25-HC for 24 hours. The luciferase activities were measured with the Bright-Glo luciferase assay system. Results were obtained from 3 independent experiments and are expressed as the means ± SEM. Statistical significance was determined by one-way ANOVA followed by Dunnett’s test. *P<0.05, **P<0.01, ***P<0.001.