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Effect of fatostatin and betulin on GSK343-induced SREBP2 processing. (A) A cartoon for SREBP processing and the stage-dependent inhibitors. (B) HepG2 and PLC5 cells were treated with GSK343 for 6 h. The whole cell lysates were analyzed by Western blotting. (C and D) HepG2 and PLC5 cells were pretreated with fatostatin (C) or betulin (D) for 1 h and then exposed to GSK343 for 6 h. The whole cell lysates were analyzed by western blotting.
