Table 4.
Upstream regulator analysis by IPA for the overlapped gene profile that is altered in GSK343-treated HepG2 and PLC5 cells
| Upstream Regulator | Description | Downstream Genes | Predicted Activity | Activation z-score | p-value |
|---|---|---|---|---|---|
| SREBF2 | Sterol regulatory element binding transcription factor 2 | ACSS2, CYP51A1, DHCR7, EBP, ELOVL6, FDPS, HMGCR, HMGCS1, IDI1, LSS, MSMO1, MVD, NSDHL, PCSK9, SC5D, SCD, SQLE, STARD4 | Activated | 4.063 | < 0.0001 |
| SCAP | SREBP-cleavage-activating protein | ACSS2, CYP51A1, DHCR7, ELOVL6, FDPS, HMGCR, IDI1, LSS, MSMO1, MVD, NSDHL, PCSK9, SC5D, SCD, SQLE, STARD4 | Activated | 3.959 | < 0.0001 |
| SREBF1 | Sterol regulatory element binding transcription factor 1 | ACSS2, CYP51A1, DHCR7, ELOVL6, FADS1, FDPS, HMGCR, HMGCS1, IDI1, LPIN1, LSS, MSMO1, MVD, NSDHL, PCSK9, SC5D, SCD, SQLE, STARD4 | Activated | 4.238 | < 0.0001 |
| POR | P450 (cytochrome) oxidoreductase | ACAT2, ASNS, CYP1A1, CYP51A1, DHCR7, ELOVL6, FDPS, HMGCR, HMGCS1, IDI1, LSS, MSMO1, MVD, NSDHL, SC5D, SCD, SQLE, STARD4 | Inhibited | -4.084 | < 0.0001 |
| INSIG1 | Insulin-induced gene 1 | ACSS2, CYP51A1, DHCR7, ELOVL6, FADS1, FDPS, HMGCR, HMGCS1, IDI1, LPIN1, LSS, SCD, SQLE, STARD4 | Inhibited | -3.654 | < 0.0001 |
Top 5 upstream regulators were shown.