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Mean differences in the fetal fraction according to library concentration (ng/μl). Model 1: Crude model. Model 2 was adjusted for GA and BMI. Model 3 added the average cfDNA size to Model 2. Model 4 added maternal age, multiple gestations, cfDNA concentration, and uniquely mapped reads to Model 2. Model 5 added the average cfDNA size to Model 4. Compared with Model 1, additional adjustment for confounding factors slightly reduced fetal fraction differences among different groups.
