Figure 5.

Relationship between fetal fraction and uniquely mapped reads. A. There was a slight trend toward higher fetal fraction with increasing uniquely mapped reads (R² = 0.001, P = 0.21). B. The mean fetal fractions on uniquely mapped reads < 1.88, 1.88-2.20, 2.21-2.56, and > 2.56 Mb were 28.6%, 30.4%, 32.0%, and 31.7%, respectively. C. In pregnant women with the same uniquely mapped reads range, fetal fraction decreased with longer cfDNA fragment size. In the same cfDNA fragment size range, fetal fraction increased with uniquely mapped reads.