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Western blot assay for P-Occludin, ROCK1 and P-MLC protein expression. The Western blot was performed at 4, 8 and 12 weeks after administration. The relative expression of P-Occludin, ROCK1 and P-MLC was displayed in (A-C) respectively. (D) Western blot assay. *P<0.05, **P<0.01 vs. CON group; #P<0.05, ##P<0.01 vs. 8 w DM group.
