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. 2019 Oct 15;11(10):6487–6497.

Figure 2.

Figure 2

LncRNA XIST regulates SOX7 expression by sponging miR-485-3p. A. The schematic diagram shows the sequences of XIST 3’-UTR wild-type (WT) and mutant type (Mut), with miR-485-3p. B. Relative expression levels of miR-485-3p in HBMEC were determined by RT-qPCR under hypoxia stimulation for various time. C. Upregulation or downregulation of miR-485-3p was achieved by transfection with miR-485-3p mimics or inhibitors in HBMEC and the efficiency were validated by RT-qPCR. D. Luciferase reporter assay revealed the luciferase activity of XIST and miR-485-3p. E. HBMEC was transfected with si-NC or si-XIST and the expression of miR-485-3p was measured. F. The schematic diagram shows the sequences of SOX7 3’-UTR WT and Mut, with miR-485-3p. G. Relative expression levels of SOX7 were determined in HBMEC under hypoxia stimulation for various time. H. Luciferase reporter assay revealed the luciferase activity of miR-485-3p and SOX7. I. Relative expression of SOX7 mRNA in HBMEC with or without XIST silencing. J. HBMEC was transfected with miR-485-3p mimics, or miRNA negative controls (miR-NC) and SOX7 mRNA expression were measured. K. SXO7 protein levels was detected in HBMEC transfected with si-XIST, miR-485-3p inhibitors with or without hypoxia stimulation. *P<0.05, **P<0.01.