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. 2019 Oct 15;11(10):6487–6497.

Figure 3.

Figure 3

XIST exerts its role in HBMEC via regulation of miR-485-3p/SOX7 pathway. (A) HBMEC were exposed to siRNA negative control (si-NC) or si-XIST and/or to miR-485-3p inhibitors for 48 h and then subjected to hypoxia for 24 h, and then cell proliferation was measured using CCK8 kit. After the above indicated treatment, wound healing assay (B), transwell assay (C) and tube formation assay (D) were performed in HBMEC and the representative images were shown in (E). (F) HBMEC were exposed to control plasmid NC (vector) or SOX7 plasmid for 48 h, and Western blot analysis of the protein expression of SOX7 was qualified. (G) HBMEC were exposed to si-NC or si-XIST and/or to SOX7 plasmid for 48 h and then subjected to hypoxia for 24 h, and cell proliferation was determined by CCK8 kit. After the above indicated treatment, transwell assay (H) and tube formation assay (I) were performed in HBMEC and the representative images were shown in (J). *P<0.05, **P<0.01.