Figure 1.

Identified seabream and sea bass granzymes are evolutionary conserved. (A) Mature peptide protein sequences of gilthead seabream, European sea bass, human and mouse GzmA, and GzmB were annealed. Dots represent conservation to the human GzmA. Gray boxes denote the conserved Cys and asterisks the catalytic triad residues. Numbers on top the sequence is based on the human chymotrypsin sequence. (B) Phylogenetic tree was constructed with protein sequences of the mature granzymes by the Neighbor-Joining method. Genetic distances were calculated based on protein differences (p-distance) with partial deletion option. The number at each node indicates the percentage of bootstrapping after 1,000 replications. Accession numbers for each sequence are indicated.