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. 2019 Oct 31;10:2579. doi: 10.3389/fimmu.2019.02579

Figure 3.

Figure 3

Fish leucocytes show granzyme activities being tryptase activity the highest during CMC reaction. (A) Gilthead seabream and European sea bass isolated head-kidney leucocytes (HKLs) were lysed and incubated with specific substrates to evaluate the tryptase, aspartase, metase, and chymase activities by colorimetry. Bars represent the mean ± SEM (n = 5). t-Student statistical differences between fish species are denoted at P ≤ 0.05 with asterisks. (B–E) Seabream and sea bass HKLs alone or in CMC assays against mock- (CMC) or NNV-infected (CMC_NNV) E-11 target cells were assayed for tryptase (B), aspartase (C), metase (D), or chymase (E) activities. Bars represent the mean ± SEM (n = 5). Different letters denote significant differences based on the ANOVA and Tukey's comparison on means test at P ≤ 0.05.