Figure 6.

Number of GzmB positive cells and area increased in the seabream brain upon NNV infection. Representative sections of brain from gilthead seabream specimens mock- (A–C) or NNV-infected during 7 days (D,E,H) or 15 days (F,G,I) subjected to immunohistochemistry (IHC) using an anti-serum against seabream GzmB (A-G) or stained with hematoxylin-eosin (H,I). Sections immunostained with anti-Gzm B serum (A,D–F), with the pre-immune serum (B) or with pre-absorbed immune serum (C,G) are shown. Representative images of the histopathological features observed in the brain upon 7 (H) and 15 (I) days of NNV infection. Black arrow heads point to the necrotic areas of the brain. Mock, control; NNV, infected with nodavirus.