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. 2019 Oct 31;10:2563. doi: 10.3389/fimmu.2019.02563

Figure 8.

Figure 8

AdV-260 and AdV-451 vaccination reduces inflammatory cytokine production, protects mice from CHIKV-induced footpad swelling, and increases survival in Ifnar−/− mice. (A) Mice were vaccinated and boosted with AdV-260, AdV-451, or AdV-Control (i.m.) followed by 1,000 PFU CHIKV challenge in the right footpad. (B) Footpad swelling was measured for 13 days after CHIKV footpad challenge. Statistics were performed using Dunnett's multiple comparison test (*p < 0.05; **p < 0.005; ***p < 0.0005; n = 7) (C) Virus was measured in the ipsilateral footpad by qRT-PCR at 8 and 14 dpi. Statistical analysis was performed on log-transformed data using Dunnett's multiple comparison test. (D) Ipsilateral ankle cytokine and chemokines were measured at 4 dpi using a 26-plex mouse cytokine and chemokine kit. Statistical analysis was performed using Kruskal-Wallis test followed by Dunn's multiple comparison post-test (*p < 0.05; **p < 0.005; n = 4). (E) Ifnar−/− mice were vaccinated and boosted with either AdV-Control or both AdV-260 and AdV-451. Mice were challenged with 1,000 PFU CHIKV in the footpad. (F) Survival of Ifnar−/− mice vaccinated with AdV-Control or AdV-260 and AdV-451 and challenged with CHIKV. Statistics were performed using Log-rank test (*p < 0.05; n = 10).