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. 2019 Nov 6;10:5033. doi: 10.1038/s41467-019-13034-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The effect of EGFL9 on cell motility in vitro. a The ectopic expression of EGFL9 does not change cell proliferation in the HMLE cell line. Cell proliferation was measured by MTT assay over 9 days. b The ectopic expression of EGFL9 increased cell migration (left panel, ***P < 0.001) and invasion (right panel, **P < 0.01) in HMLE cells. c The ectopic expression of EGFL9 does not change cell proliferation in the EpRas cell model. Cell proliferation was measured by MTT assay over 7 days. d The ectopic expression of EGFL9 increased cell migration (left panel, **P < 0.01) and invasion (right panel, **P < 0.01) in EpRas cells. e Knockdown of EGFL9 expression does not affect the cell proliferation in 4T1 cells. Cell proliferation was measured by MTT assay over 6 days. f Knockdown of EGFL9 expression significantly decreased migration (left panel, shRNA2/non-target control = 21% and shRNA3/non-target control = 12%, ***P < 0.001) and invasion (right panel, shRNA2/non-target control = 19% and shRNA3/non-target control = 5.5%, ***P < 0.001) in 4T1 cells. g Knockdown of EGFL9 expression does not affect proliferation of SUM159 cells. Cell proliferation was measured by MTT assay over 7 days. h Knockdown of EGFL9 expression decreased migration (left panel, shRNA1/non-target control = 22.2% and shRNA3/non-target control = 21%, ***P < 0.001) and invasion ability (right panel, shRNA1/non-target control = 32% and shRNA3/non-target control = 16%, ***P < 0.001) in SUM159 cells. All experiments were performed in triplicate. The data number was the mean value from five randomly selected field views. Each bar represents the mean ± SEM (standard error of the mean). P values were determined by unpaired two-tailed t-test