Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 6;10:5033. doi: 10.1038/s41467-019-13034-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Physical association and co-localization of EGFL9 and cMET. a Interaction of EGFL9 and cMET in HMLE/EGFL9 cells. Left panel: immunoprecipitation was performed with anti-v5 antibody followed by immunoblotting with cMET antibody. Right panel: immunoprecipitation was performed with anti-cMET followed by immunoblotting with anti-V5 antibody. b Interaction of EGFL9 and cMET in SUM159 cells. Immunoprecipitation was performed with anti-cMET antibody followed by immunoblotting with anti-EGFL9 antibody. For both a and b, IgG was used as the IP-control. c, d Subcellular co-localization of EGFL9 and cMET in HMLE/EGFL9 (c) and SUM159 (d) cells. The distribution of ectopic expressed EGFL9 was detected with anti-v5 antibody (c). Endogenous EGFL9 was detected by EGFL9 antibody (d). cMET was detected with anti-cMET antibody (c, d). DAPI staining was included to visualize the cell nucleus. Scale bar: 10 µm. e Co-localization of EGFL9 and cMET in mitochondria in HMLE/EGFL9 cells. Mitochondria were stained with Mitotracker deep-red dye. The distribution of ectopic expressed EGFL9 was detected with an anti-V5 antibody. cMET was detected with an anti-cMET antibody. DAPI staining was included to visualize the cell nucleus. Scale bar: 10 µm. f Cytosolic and mitochondrial fractions were isolated from HMLE/EGFL9 cells and subjected to SDS-PAGE followed by probing with specific antibodies as indicated. Cyt c, mitochondrial marker; β-actin, cytosolic marker. g Proteinase K protection assay. Equal amounts of purified mitochondria were treated as indicated. Lysed protein was separated by SDS-PAGE and immunoblotted using the indicated antibodies