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. 2019 Sep 2;123(6):709–722. doi: 10.1038/s41437-019-0263-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Experimental design and mating scheme to produce and maintain full-sib inbred lines and an outbred control during five (Experiment I) or six (Experiment II) generations, and their corresponding inbreeding coefficient (F) reached. n: total number of inbred lines (50 for Exp I and 68 for Exp II. nb: Number of bottles of Base Population (30 for Exp I and 50 for Exp II). The control line is maintained with maximum avoidance of inbreeding