Fig. 6.

Cytokine concentration. Spleen was collected from each mouse and pooled within groups (n = 6) and splenocytes were isolated and cultured at 2.5 × 10⁶ cells/mL concentration of cells in triplicate wells as unstimulated (control) or stimulated with 50 μg/well of Ova for 72 h. The cytokine concentration of cell culture supernatants for IL-4, IFN-γ, IL-12, IL-10, TGF-β, and IL-17 were determined by ELISA. Different letters indicate significant difference between groups for each cytokine. Significance was taken at p ≤ 0.05