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. 2019 Nov 6;10:5035. doi: 10.1038/s41467-019-13008-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Microfluidic-induced sleep behavior depends on ALA and RIS neurons. a Raster plots of detected sleep bouts from WT, ceh-17(lf) and aptf-1(lf) animals. Only the top 50 animals showing the most total microfluidic-induced sleep are shown. b Both ceh-17(lf) and aptf-1(lf) show less total sleep than WT. The data suggest that microfluidic-induced sleep is strongly dependent on the ALA and RIS neurons. (WT n = 57, ceh-17(lf) n = 57, aptf-1(lf) n = 60; *****p < 0.00001 compared with WT, Kruskal–Wallis with a post hoc Dunn–Sidak test). Source data is available as a Source Data file