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. 2019 Nov 6;9:16144. doi: 10.1038/s41598-019-52502-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression analysis of Fe deficiency-related genes in (A) Oryza sativa ssp. spontanea; (B) O. barthii; (C) O. longistaminata; (D) O. punctata; (E) O. latifolia; (F) O. australiensis. Relative expression levels (RT-qPCR, relative to OsUBQ5 expression) of selected genes (IRT1, YSL15, IRO2, and NRAMP1) in roots of plants submitted to control (CC) or Fe deficiency (−Fe) conditions for five days. Roots were collected from plants grown in non-aerated nutrient solution, at three-leaf stage on both conditions at the time of RNA extraction. Values are the averages of four samples (3 plants each) ± SE. Asterisks indicate statistical difference between plants grown under CC and −Fe conditions (Student t-test, *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001, ****P-value < 0.0001).