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. 2019 Nov 6;9:16092. doi: 10.1038/s41598-019-52627-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased expression of fibrotic markers in siAtg5/7-transfected TM cells. (A) qPCR analysis confirming changes in expression levels of TGFβ2, BAMBI and SMA genes in siAtg5/7-transfected TM cells. (B) Levels of total TGFβ2 (latent and active) in culture media from siAtg5/7-transfected TM cells quantified by ELISA and normalized with total μg of corresponding whole cell lysates. (C) Protein levels of SMA as evaluated by western blot in whole-cell lysates (5 μg) from three independent human TM primary cells transfected for 72 h with siAtg5/7. (D) Mean SMA relative protein levels quantification as calculated from densitometric analysis of the bands, expressed as percentage of siNC controls. β-actin was used as loading control. *p < 0.05, **p < 0.01, t-test, n = 3. RPL: Relative protein levels.