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. 2019 Nov 6;9:16118. doi: 10.1038/s41598-019-52604-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell morphology. Distribution of the cell dimensions (i - length, ii - width, iii - width/length ratio and iv - height) measured with AFM for N individual cells of the studied clonal variants (blue circle - IST439, red square - IST4113 and green triangle - IST4134), for (a) planktonic and (b) biofilm growth conditions both measured in air and for the (c) planktonic form measured in liquid environment. An increase of the width/length ratio and a decrease of cell height, as well as the evolution of the cell shape during long-term infection from a rod-like to a more cocci-like morphology can be observed. The results of the Mann-Whitney u-test (^*P < 0.05, ^**P ≤ 0.005, ^****P ≤ 0.0005, ns not significant) are indicated.