Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 6;5:34. doi: 10.1038/s41522-019-0108-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — The network linking mutations with the growth regime, phenotypes, and biological replicates. The force-directed representation of the network is constructed based on co-occurrence patterns and correlations (p value <0.01) between mutations and the growth regime, between mutations and phenotypic measures, and between mutations and biological replicates. Growth regime/sample types are presented as color-filled nodes: P—planktonic culture (yellow-filled), B—antibiotic-free biofilm effluent (blue-filled), BC—ciprofloxacin-exposed biofilm effluent (red-filled), BT—tetracycline-exposed biofilm effluent (green-filled). Phenotypes are presented as color-outlined nodes: Bf (blue-outlined)—biofilm formation, measured in microtiter plate assay; Cr (red-outlined)—resistance to ciprofloxacin measured in MIC assay; and Tr (green-outlined)—resistance to tetracycline measured in MIC assay. P, B, BT, and BC nodes represent the sample type; Bf, Cr, and Tr nodes represent a phenotype; R1, R2, and R3 represent biological replicates 1, 2, and 3, respectively; all other nodes denote mutations directly linked with a specific sample type and/or phenotype. The fill color of nodes corresponds to the sample type that the mutation directly correlates with (linked to P—yellow-filled, to B—blue-filled, to BC—red-filled, to BT—green-filled). The node outline color corresponds to the phenotype with which the mutation is directly linked to (linked to Bf—blue-outlined, to Cr—red-outlined, to Tr –green-outlined). Mutations directly linked to both ciprofloxacin and tetracycline resistance are outlined in khaki. Mutations linked to a particular biological replicate are indicated as squares (linked to replicate 1), triangles (linked to replicate 2), diamonds (linked to replicate 3), pentagons (linked to replicates 1 and 2), octagons (linked to replicates 1 and 3), and a hexagon (linked to replicates 1, 2, and 3). The size of the node is relative to the node authority. Edges (the lines connecting the nodes) represent correlations between two nodes, positive correlations are presented in green, and negatives in magenta. Edge thickness/intensity represents the strength of correlation. The full description of each mutation is presented in Table 2