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Primer sequences of recombinant protein F1, F2 and F3 (restriction site is italic and underlined).
| Name The Synthesized oligonucleotides | Restriction site |
|---|---|
| F1-F: 5-CGGGATCCAGCTCCACCAAGGAGGG-3 | BamHI |
| F1-R: 5-CCCTCGAGTTCCCGCGTGGACACCT-3 | XhoI |
| F2-F: 5-CGGGATCCTCGGACTCGCCGGA-3 | BamHI |
| F2-R: 5-CCCTCGAGGTCGATGCTGTTCT-3 | XhoI |
| F3-F: 5-CGGGATCCGACGGCACGCACTAC-3 | BamHI |
| F3-R: 5-CCCTCGAGCTTGCAGAACTCCGA-3 | XhoI |
