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. 2019 Nov 6;9:16137. doi: 10.1038/s41598-019-52411-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the location of the probe sets for the multiplex Htt QuantiGene assays. Custom probe sets were designed against Htt mRNA sequence to enable the measurement of all major Htt transcripts. The 9-plex assay contained probe sets to intron 1 sequences before the first cryptic polyA site at 680 bp to detect the Httexon1 transcripts, to the 3′ end of intron 1 and to intron 3 as controls for unprocessed mRNA, and to exons 50–53 for all Htt-FL transcripts. The 10-plex assay included a probe set to sequences between the first and second cryptic polyA sites, between 680 and 1145 bp. The 14-plex assay also included probe sets to each of the three 3′UTRs of the full-length transcript.