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. 2019 Nov 6;9:16137. doi: 10.1038/s41598-019-52411-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the analysis of Htt transcripts in the striatum of zQ175 heterozygous animals using the 10-plex QuantiGene panel and qPCR assays. Expression of the Htt intronic sequences and Htt-FL transcripts was measured by our QuantiGene 10-plex assay (a–j) and qPCR (k-t) in striatum, cortex, hippocampus, cerebellum and brain stem of 2 month old mice, and presented relative to that for housekeeping genes. (a–e and k–o) Httexon1 transcripts can be detected in all brain regions by both approaches. (f–j and p–t) The levels of Htt-FL as measured by both approaches were comparable. Data were obtained from n = 4/genotype with two technical replicates for QuantiGene and three for qPCRs. Statistical analysis was by multiple t-test with Benjamini-Hochberg with an alpha of 0.05; *p < 0.05, **p < 0.01, ***p < 0.001. Tissue lysate dilutions were 1:3 for striatum and brain stem, 1:4 for cortex and cerebellum and 1:5 for hippocampus.