Table 1.

Design of the 9-plex and 10-plex QuantiGene assays for the detection of Htt transcripts.

Transcript/gene symbol	Transcript/gene name	GOI/HK	9-plex	10-plex
(Htt)-I1-pA1	Huntingtin – Intron 1- poly(A)1	Htt	√	√
(Htt)-I1-pA2	Huntingtin – Intron 1- poly(A)2	Htt	X	√
(Htt)- I1-3’	Huntingtin – Intron 1–3’	Htt	√	√
(Htt-)-I 3	Huntingtin – Intron 3	Htt	√	√
Htt-FL (E50-53)	Huntingtin – exons 50–53	Htt	√	√
Eif4a2	Eukaryotic Initiation factor 4a2	HK	√	√
Rpl13a	Ribosomal protein L 13a	HK	√	√
Ubc	Ubiquitin C	HK	√	√
CanX	Calnexin	HK	√	√
Atp5b	ATP synthase subunit beta	HK	√	√

GOI = gene of interest, HK = housekeeping reference gene.