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. 2019 Nov 6;10(11):843. doi: 10.1038/s41419-019-2053-8

Fig. 6. FOXO3a plays critical roles in doxorubicin-induced autophagy and chemosensitivity of HCC cells.

Fig. 6

a Relative cell viability (mean ± SD) for FOXO3a or NC siRNA transfected HepG2, HuH-7, SNU-387, and SNU-449 cells treated with doxorubicin for 48 h. b LC3 and p62 protein expression in control HCC cells and HCC cells transfected with FOXO3a siRNA or NC siRNA followed by doxorubicin treatment for 48 h. c Ultrastructural features of intracellular double-membrane vesicles in control HCC cells and HCC cells transfected with FOXO3a siRNA or NC siRNA followed by doxorubicin treatment, counterstained with 4% uranyl acetate and observed by TEM. Scale bar = 10 μm. d Up, mRFP-GFP-LC3 stable HepG2, HuH-7, SNU-387, and SNU-449 cells in the untreated groups or groups with FOXO3a siRNA or NC siRNA transfection followed by doxorubicin treatment for 48 h were visualized by confocal microscopy. Down, number of GFP+/mRFP+-LC3 (yellow) and GFP−/mRFP+-LC3 (red) dots were scored on 50 cells. ($$$p < 0.001, ***p < 0.001, Control vs. NC siRNA plus doxorubicin treatment; @@@p < 0.001, ###p < 0.001, NC siRNA plus doxorubicin treatment vs. FOXO3a siRNA plus doxorubicin treatment)