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. 2019 Nov 6;10(11):843. doi: 10.1038/s41419-019-2053-8

Fig. 7. miR-223 directs doxorubicin-induced autophagy in HCC cells by targeting FOXO3a.

Fig. 7

a Relative cell viability (mean ± SD) for FOXO3a siRNA transfected HepG2, HuH-7, SNU-387 and SNU-449 cell lines, followed by miR-223 or NC inhibitor transfection and doxorubicin treatment for 48 h. b FOXO3a, LC3, and p62 protein expression in control HCC cells and FOXO3a siRNA transfected HCC cell lines, followed by miR-223 or NC inhibitor transfection and doxorubicin treatment for 48 h. c Ultrastructural features of intracellular double-membrane vesicles in FOXO3a siRNA transfected HCC cell lines followed by miR-223 or NC inhibitor transfection and doxorubicin treatment, counterstained with 4% uranyl acetate and observed by TEM. Scale bar = 10 μm. d Left, mRFP-GFP-LC3 stable HepG2, HuH-7, SNU-387, and SNU-449 cells in FOXO3a siRNA transfected groups followed by miR-223 or NC inhibitor transfection and doxorubicin treatment for 48 h were visualized by confocal microscopy. Right, number of GFP+/mRFP+-LC3 (yellow) and GFP−/mRFP+-LC3 (red) dots were scored on 50 cells. (n.s. p > 0.05, FOXO3a siRNA plus doxorubicin treatment vs. miR-223 inhibitor plus FOXO3a siRNA plus doxorubicin treatment)