Fig. 3.

IL-27R deficiency leads to limited expansion of HSCs in response to Ang II. BM was obtained from Apoe^−/−Il27ra^+/− (n = 7) and Apoe^−/−Il27ra^−/− (n = 5), or Apoe^−/−Il27ra^+/− (n = 12) and Apoe^−/−Il27ra^−/− (n = 12) mice fed with WD for overall period of 12 weeks and infused with PBS or Ang II for last 4 weeks of feeding, respectively. a Representative dot plots of live LSK (Lin⁻Sca-1⁺c-kit⁺) and myeloid progenitors (Lin⁻Sca-1⁻c-kit⁺). b Representative dot plots of live LT-HSC (Lin⁻Sca-1⁺c-kit⁺CD150⁺CD48⁻) and HPC-1 (Lin⁻Sca-1⁺c-kit⁺CD48⁺CD150⁻) cells. c Average percentage of live LSK, LT-HSC, HPC-1, myeloid progenitors, and CMP (Lin⁻Sca-1⁻c-kit⁺CD16/CD32⁻CD34⁺). d Absolute number of live LSK, LT-HSC, HPC-1, myeloid progenitors, and CMP. e Proliferation of HSPCs from Apoe^−/−Il27ra^+/− (n = 8) or Apoe^−/−Il27ra^−/− (n = 6) mice fed with WD and infused with Ang II or PBS as determined by BrdU incorporation. Percentage of live BrdU-positive LSK and myeloid progenitors, including LT-HSC, HPC-1, and CMP. Data are mean ± SEM from two independent experiments. f Percentage of progenitor cells from Apoe^−/−Il27ra^+/− (n = 6) or Apoe^−/−Il27ra^−/− (n = 6) mice infused with Ang II in G₀/G₁ and G₂/M phase of cell cycle, analyzed for BrdU inclusion, and DAPI staining. Data are mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005, unpaired Student’s t-test (two-tailed)