Fig. 3. BRG1 regulates CCNB1 transcription in lung cancer cells.

a A CCNB1 promoter-luciferase construct was transfected into LLC cells with or without BRG1 followed by exposure to 1% O₂ for 24 h. Luciferase activities were normalized by both protein concentration and GFP fluorescence. b CCNB1 promoter-luciferase constructs of different lengths were transfected into LLC cells with or without BRG1 followed by exposure to 1% O₂ for 24 h. Luciferase activities were normalized by both protein concentration and GFP fluorescence. c Wild type and mutant CCNB1 promoter-luciferase constructs were transfected into LLC cells with or without BRG1 followed by exposure to 1% O₂ for 24 h. Luciferase activities were normalized by both protein concentration and GFP fluorescence. d HA-tagged BRG1 and FLAG-tagged E2F1 were transfected into HEK293 cells. Co-immunoprecipitation was performed with indicated antibodies. e LLC cells were transfected with siRNA targeting E2F1 or SCR before exposure to 1% O₂ for 24 h. ChIP assays were performed with anti-BRG1 or IgG. Inset, knockdown efficiency of E2F1. f LLC cells were exposed to 1% O₂ and harvested 24 h later. Re-ChIP assay was performed with indicated antibodies